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Mechanism of Vesicles Docking and Fusion

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Abstract:  Analysis of Biochemical and Genetics study reveal a molecular mechanism which is selectively cargo molecule transport between donor and target compartments .                                        Different membrane proteins participate in vesicular fusion .         #We will discuss about some membrane proteins which participate in vesicles docking and fusion . Protein involved in vesicle docking 1: SNAREs protein: Complimentary sets of vesicles SNAREs(v-SNARE) and target membrane (t-SNARE) contribute to the selectivity of transport vesicle docking and fusion.   v-SNARE: During the budding of transport vesicles it is packaged together with coat protein from donor membrane and then bind to complementary t-SNARE in target membrane.   t-SNARE: After the fusion t-SNARE Mechanism and v-SNARE make tight co...
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Recombinant DNA technology ( Alpha complementation and Blue white screening)

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Recombinant DNA Technology including Blue white screening and Alpha complementation. What is gene ? It is segment of DNA which express is specific type of protein. What is cloning? The exact genetic copies To produce multiple copies of gene we use gene cloning technology or techniques. There are some methods of gene cloning -- method for molecular cloning using polymerase chain reaction and cell free gene cloning method If we use any host cell to make multiple copies of our gene of interest then this type of methods are called cell based gene cloning method.
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The journey to the discovering of DNA : A molecule changed the world

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Abstract: If I ask you ,what is DNA ...? You may reply that it is a hereditary material found in almost every organism,contains the genetic instructions of organism for its development or functions...etc. But today you could learn a history of DNA .  I just tell you to pack your excitement to the top and let's know something new.  The Discovery of DNA : There are so many people who had already guess that the discovery of the DNA was done by Watson and Francis Crick but it is fully wrong . In 1860s ,a Swiss chemist ,Johann Friedrich Miescher unknowingly discovered DNA . One day Johann planned to research on white blood cells' key components .He collected pus coated bandages from a nearby medical clinic . Johann use salt solution to understand more about WBC and further he  added acid to the solution . Then a substance separated from the solution. Next he added alkali and it dissolved again .However during this process he understood that the substance has an u...
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