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Mechanism of Vesicles Docking and Fusion

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Abstract:  Analysis of Biochemical and Genetics study reveal a molecular mechanism which is selectively cargo molecule transport between donor and target compartments .                                        Different membrane proteins participate in vesicular fusion .         #We will discuss about some membrane proteins which participate in vesicles docking and fusion . Protein involved in vesicle docking 1: SNAREs protein: Complimentary sets of vesicles SNAREs(v-SNARE) and target membrane (t-SNARE) contribute to the selectivity of transport vesicle docking and fusion.   v-SNARE: During the budding of transport vesicles it is packaged together with coat protein from donor membrane and then bind to complementary t-SNARE in target membrane.   t-SNARE: After the fusion t-SNARE Mechanism and v-SNARE make tight co...
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The Cell Membrane: Movement of phospholipids in Lipid bilayer

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Abstract : Phospholipid is a major component which show different types of movements. Here we will describe 5 types of movement of lipid in lipid bilayer. So don't be late ..       Lets learn.... https://pin.it/2J6aICm Lateral diffusion Rotation Swing Flexion 5.Flip-Flop/Transverse diffusion.        As a component we took phospholipid to show you the procedure very easy way . #1 : Lateral diffusion : Cell membrane molecules transpose with it's local molecules . Lateral movement also provide cell membrane fluid structure. It has been discovered that phospholipid moves via hop diffusion. #2:  Rotation: It occurs when a lipid molecule rotates itself very fast around it's axis and interact with intermidiate neighbor molecules. #3:   Swing: Molecules swing from one side to another one. #4: Flexion : The movement is started by contraction.  #5: Flip-Flop movement/Transverse movement: However Flip-...
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Recombinant DNA technology ( Alpha complementation and Blue white screening)

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Recombinant DNA Technology including Blue white screening and Alpha complementation. What is gene ? It is segment of DNA which express is specific type of protein. What is cloning? The exact genetic copies To produce multiple copies of gene we use gene cloning technology or techniques. There are some methods of gene cloning -- method for molecular cloning using polymerase chain reaction and cell free gene cloning method If we use any host cell to make multiple copies of our gene of interest then this type of methods are called cell based gene cloning method.
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