Vesicle formation of Cop I and Cop II

-

Learn how COP I and COP II vesicle formed in diagram in step by step.

Abstract: It is very interesting to know how COP I & COP II vesicles formed when Retrograde and Anterograde transport occur.... Today we will highlight on the mentioned matter.

Lets go..

#An imaginary picture of anterograde and retrograde.




  • Endoplasmic Reticulum resident protein has KDEL sequence at the protein c terminal end .
  • KDEL receptor present in Golgi.
  • KDEL receptor recognise ER resident protein and transport the protein to ER (Retrograde transport) by COP I .
ER transport their protein by vesicular transport by using COP II to Golgi.

Formation of COP I coated vesicle:
1.Recruitment of Sec7 (GEF) .
2. After recruitment of ARF1 from cytosol, Sec7  converts GDP to GTP of ARF1 and make it active from inactive form.
3.Hydrophobic tail present in ARF1 GTP which inserted into golgi membrane. 
4.Finally COP I is activated by ARF1 .
5.As the picture before fusion the vesicle COP I to ER membrane, activated ARF1 will hydrolysis and convert itself  GTP toGDP again.
6.Next COP I  will dissociate and the vesicle simply fuse with ER membrane by the help of Rab effector , v-SNARE and t-SNARE (SEARCH IN MY WEBSITE TO KNOW)







Formation of COP II coated Vvesicle:

The process is quite same as COP I.
1.Here recruitment of Sec12 (GEF) 
GEF: Guanine nucleotide Exchange Factor
It then activates Sar1(GDP to GTP)
2.Sar1 gives signal to COP II to which make vesicle formation .
3.sar 1 hydrolysis in GTP to GDP again.
4.COP II dissociate and fused with Golgi .


My website link: subhadipzoolover.blogspot. com

Thank you ...
          If my article help you put a comment and subscribe "Biology Extrema".....

Comments

Post a Comment

Popular posts from this blog

Mechanism of Vesicles Docking and Fusion

-
Image
Abstract:  Analysis of Biochemical and Genetics study reveal a molecular mechanism which is selectively cargo molecule transport between donor and target compartments .                                        Different membrane proteins participate in vesicular fusion .         #We will discuss about some membrane proteins which participate in vesicles docking and fusion . Protein involved in vesicle docking 1: SNAREs protein: Complimentary sets of vesicles SNAREs(v-SNARE) and target membrane (t-SNARE) contribute to the selectivity of transport vesicle docking and fusion.   v-SNARE: During the budding of transport vesicles it is packaged together with coat protein from donor membrane and then bind to complementary t-SNARE in target membrane.   t-SNARE: After the fusion t-SNARE Mechanism and v-SNARE make tight co...
Read more

Recombinant DNA technology ( Alpha complementation and Blue white screening)

-
Image
Recombinant DNA Technology including Blue white screening and Alpha complementation. What is gene ? It is segment of DNA which express is specific type of protein. What is cloning? The exact genetic copies To produce multiple copies of gene we use gene cloning technology or techniques. There are some methods of gene cloning -- method for molecular cloning using polymerase chain reaction and cell free gene cloning method If we use any host cell to make multiple copies of our gene of interest then this type of methods are called cell based gene cloning method.
Read more

Marine Life

-
Image
MARINE LIFE 95% of our ocean have not been explored yet; it is hard not to let your imagination run wild. But thanks to brave explorers deep sea cameras and awesome archaeologist; we do know about some pretty incredible sea creatures living in our waters today from millions of years ago … Life on earth is supported by the surrounding ecological conditions and the natural resources. Continents and oceans are the two major divisions, comprising of all the life and also the ecosystems throughout the world. About 75 % of the earth’ surface is covered with oceanic waters which has a very rich wealth of marine life. The ocean is a major source of food, energy, and mineral resources. Oceans also control the global climate. If we say that land is dominated by plant biomass, then we have to say that animal biomass dominates the oceanic waters. Water-based living environments are called as aquatic ecosystems. Aquatic Ecosystems are classified into two types as freshwater ecosystems and marine ...
Read more